Skip to main content
. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Feb 5;432(1):10–15. doi: 10.1016/j.bbrc.2013.01.098

Fig. 1.

Fig. 1

Potential partner proteins interact specifically with GIP in a yeast two-hybrid assay and internal motifs in newly-identified hGIP partners. (A) The two-hybrid reporter yeast strain AH109 was co-transformed with plasmids expressing the Gal4AD-fusion protein indicated at left and either human GIP or ADA3 fused to Gal4BD. Representative co-transformants were streaked on selective medium (SD/-Ade/-His/-Trp/-Leu/X-α-Gal) and incubated at 30°C. Growth and expression of the alpha-galactosidase reporter gene (blue color) indicates interaction of the co-expressed fusion proteins. (B) The carboxy-terminal sequence of the two new protein partners with the potential internal GIP recognition motifs indicated by grey boxes.