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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Feb 3;20(3):317–325. doi: 10.1038/nsmb.2499

Figure 6. HDACi or 53BP1 deficiency restores end resection and homology directed DSB repair in TIP60 deficient cells.

Figure 6

(a) IF was performed with antibodies to RPA or Rad51 in siRNA transfected U2OS-DSB-reporter cells that were incubated with either DMSO control or TSA (0.5 μM). Scale bar, 10 μm. (b, c) Quantification of RPA and Rad51 DSB localization from a. Analysis was performed on 200 cells per group in at least 2 independent experiments. Error bars represent S.E.M. * p < 0.05, n.s. not significant. (d) Homology directed repair of I-SceI DSBs in siRNA transfected U2OS DR-GFP reporter cells as assessed by FACS for percent GFP positive cells. *p<0.05, n.s. = not significant.