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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Feb 3;20(3):317–325. doi: 10.1038/nsmb.2499

Figure 7. 53BP1 promotes genomic instability and sensitivity to PARPi in the absence of TIP60.

Figure 7

(a) Representative metaphase images from 53BP1+/+ and 53BP1−/− MEFs following knockdown of TIP60 and Olaparib treatment. Significance was calculated using Fisher’s exact test. (b) Percentage survival following Olaparib administration in U2OS cells that had been transfected with the indicated siRNAs. (c) Model depicting that TIP60 -dependent acetylation limits 53BP1 binding to H4K20Me2 when present on the same histone H4 tail. TIP60 deficiency would result in hypoacetylated H4K16 and a 53BP1-dependent block of BRCA1 DSB chromatin association.