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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2012 Nov 29;85(4):1119–1126. doi: 10.1016/j.ijrobp.2012.09.015

Figure 1. Induction of apoptosis in LS-174T xenografts model.

Figure 1

A. Treatment Schedule. Mice bearing i.p. LS-174T xenografts were treated with Gem followed by 212Pb-TCMC-trastuzumab at the indicated time points. Additional groups included untreated, Gem alone, 212Pb-TCMC-trastuzumab, and 212Pb-TCMC-HuIgG with/without Gem pre-treatment.

B. Apoptosis induced by 212Pb-TCMC-trastuzumab in response to Gem. The apoptotic nuclei at the indicated time points were counted under light microscopy; 500 nuclei were scored per tumor. Results represent the average of a minimum of three replications (± SD).

C. Fluorescence microscopy images of apoptosis at 24 h. Paraffin-embedded sections at 24 h were stained with TUNEL. Top, TUNEL staining; bottom, DAPI counter staining (40x)