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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Protein Expr Purif. 2013 Jan 11;88(2):196–200. doi: 10.1016/j.pep.2013.01.003

Figure 1.

Figure 1

Expression construct for PAf39 peptide. Panel A. The hexahistidine tagged-ubiquitin gene with a TEV protease cleavage site followed by the PAPf39 coding sequence was inserted into a pTXB1 construct to yield the pTXB1::6H_UBQ_TEV_PAPf39_INTEIN expression plasmid. Panel B. The sequence of the expressed construct: residues 1-83 correspond to the N-terminal 6H-tagged ubiquitin, residues 84-90 comprise the TEV protease recognition motif (cleavage between Q89 and G90), residues 90 to 128 correspond to the PAPf39 peptide, and residues 129 through 388 correspond to the intein sequence.