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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Biol Trace Elem Res. 2013 Jan 15;152(1):113–116. doi: 10.1007/s12011-012-9597-0

Figure 1.

Figure 1

The effect of 4-PAS on MTT reduction (A) and membrane integrity (B) in RBE4 cells cultured in 96-well culture plates treated for 24 h with 400, 600 or 800 μM MnCl2 or medium alone. After 24 h RBE4 cells were treated for 45 minutes with medium or 1 or 2 mM 4-PAS in DMSO. Values of MTT (% control) (A) and LDH (% control) (B) represent mean ± SEM (n=12). ^^^ p<0.0001 significantly different from control group, *** p<0.0001,** p<0.001 significantly different from RBE4 cells exposed to the respective Mn 400, 600 and 800 μM group by one-way ANOVA followed by Bonferroni’s multiple comparison tests.