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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jan 26;1830(4):2981–2988. doi: 10.1016/j.bbagen.2013.01.018

Figure 5. Attenuation of nuclear translocation of NF-κB p65 by NOE and SPE in RAW 264.7 macrophage.

Figure 5

Cells were pre-treated with 50 μg/mL of NOE or SPE for 12 h and subsequently they were activated by 100 ng/mL LPS for 1 or 2 h. Cytoplasmic and nuclear fractions were isolated and Western blot analysis for p65 was conducted. TATA binding protein (TBP) and GAPDH were used as a nuclear and cytoplasmic marker, respectively.