Table 2.
Compound | Structure | IC50 ± SEM (μM) |
||
---|---|---|---|---|
recombinant Ndh | Mtb H37Rv (aerobic) | Mtb H37Rv (Wayne model) | ||
Chlorpromazine | 70.1 ± 9.6 | 23.7 ± 4.2 | 11.7 ± 2.5 | |
Flupenthixol | 28.6 ± 1.7 | 13.2 ± 1.2 | 10.0 ± 0.3 | |
Fluphenazine | 47.8 ± 3.2 | 14.5 ± 2.3 | 3.4 ± 0.9 | |
Perphenazine | 47.5 ± 6.6 | 18.8 ± 3.6 | 6.8 ± 1.4 | |
Phenothiazine | >200 | >100 | >100 | |
Promazine | 155.1 ± 17.3 | 62.1 ± 5.7 | 10.0 ± 3.8 | |
Promethazine | 85.3 ± 16.1 | 50.8 ± 1.6 | 22.6 ± 4.8 | |
Thioridazine | 11.4 ± 1.8 | 13.4 ± 2.1 | 3.9 ± 0.8 | |
Trifluoperazine | 15.6 ± 2.2 | 10.5 ± 1.6 | 3.6 ± 0.7 |
Values were calculated spectrophotometrically (recombinant Ndh) or using the MABA assay (Mtb), as described in the Materials and methods section, and are the mean of at least two replicates.