Table 1.
Near infrared fluorophores used in this study
| Dye | Abs max (nm) |
Em max (nm) |
Extinction coefficient1 (M−1cm−1) |
F/P | Qr | Brightness (CV)2 |
|---|---|---|---|---|---|---|
| ALEXA 750 | 749 | 775 | 260,000 | 2.8 | nd | 150,354 (17%) |
| IRDye800 | 774 | 789 | 300,000 | 2.5 | 2.4 | 85,333 (15%) |
| DyLight 800 | 777 | 794 | 270,000 | 1.5 | 0.5 | 56,046 (8%) |
| Cy7.5 | 788 | 808 | 223,000 | 0.1 | 0.6 | 66,895 (15%) |
| ALEXA 790 | 785 | 810 | 260,000 | 0.4 | 1.2 | 77,805 (21%) |
| NIR797 | 795 | 817 | 94,100 | 2.7 | 0.6 | 153,402 (19%) |
| Dylight 830 | 730 | 830 | 220,000 | nd | nd | 25,452 (16%) |
1
Provided by manufacturer.
2
Median integrated emission.
nd, not determined