Table 2.
Functional analysis of interactions of the distal CTD
| PLCβ3 variant | Basal specific activity ± SEM (nmol IP3/min/ nmol PLCβ3) (n)b |
Gαq variant | Ki ± SEM (nM) (n)b |
EC50 ± SEM (nM) |
Max specific activity ± SEM (nmol IP3/min/nmol PLCβ3) (n)b |
|---|---|---|---|---|---|
| PLCβ3 | 54 ± 2.7* | Gαq | 220 ± 30 (24) | 120 ± 30 | 2900 ± 130 (32) |
| -ΔN | 270 ± 110 (9) | 39 ± 1 | 1600 ± 100 (6) | ||
| -K311A | 260 ± 39 (4) | 130 ± 21 | 2900 ± 360 (10) | ||
| -D296A/P298D | 270 ± 23 (3) | 430 ± 110 | 3400 ± 400 (12) | ||
| -C9S/C10S | NDa | 90 ± 11 | 1800 ± 190 (6) | ||
| PLCβ3 L876A | 950 ± 69* | Gαq | 20 ± 3.2 (11) | 19 ± 2 | 6500 ± 460 (6) |
| -ΔN | 24 ± 7.7 (7) | 5 ± 1 | 2800 ± 250 (6) | ||
| -K311A | 16 ± 3.9 (3) | 14 ± 2 | 4400 ± 150 (4) | ||
| -D296A/P298D | 12 ± 3.4 (4) | 27 ± 3 | 6800 ± 640 (5) | ||
| -C9S/C10S | NDa | 40 ± 4 | 3900 ± 440 (6) | ||
| PLCβ3-Δ892 | 4.7 ± 0.6* | Gαq | 490 ± 66 (14) | 59 ± 7 | 39 ± 2 (6) |
| -ΔN | 490 ± 130 (6) | 59 ± 7 | 31 ± 1 (6) | ||
| -K311A | 380 ± 70 (3) | 150 ± 20 | 48 ± 3 (6) | ||
| -D296A/P298D | 420 ± 88 (6) | 540 ± 250 | 40 ± 4 (8) | ||
| -C9S/C10S | NDa | 150 ± 20 | 29 ± 2 (6) | ||
| PLCβ3 EEE | 46 ± 7 (9) | Gαq | 93 ± 23 (5) | 280 ± 130 | 1900 ± 170 (5) |
| -ΔN | 78 ± 26 (3) | 120 ± 30 | 2000 ± 150 (6) | ||
| PLCβ3 L876AEEE | 730 ± 63 (10) | Gαq | 26 ± 9.1 (4) | 32 ± 20 | 3900 ± 1300 (4) |
| -ΔN | 42 ± 30 (4) | 9 ± 3 | 3400 ± 680 (6) | ||
| PLCβ3 T652E | 5.0 ± 0.5 (6) | Gαq | 160 ± 38 (5) | 1150 ± 790 | 460 ± 170 (6) |
| PLCβ3-Δ892 T652E | 1.0 ± 0.1 (7) | Gαq | 250 ± 88 (3) | 250 ± 80 | 13 ± 2 (4) |
| PLCβ3-Δ882-937 | 200 ± 8 (7) | Gαq | 230 ± 100 (3) | NDa | 200 ± 20 (6) |
| PLCβ3-Δ882-937 L876A | 500 ± 95 (9) | Gαq | 12 ± 2.3 (3) | NDa | 450 ± 25 (5) |
1
Previously reported19.
a
Not Determined.
b
Number (n) of independent experiments conducted in duplicate.