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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Curr Opin Chem Biol. 2012 Nov 10;17(1):90–101. doi: 10.1016/j.cbpa.2012.10.034

Figure 5. Production of O-GlcNDAz requires chemical synthesis and enzyme engineering.

Figure 5

Diazirine-modified, cell-permeable GlcNAc-1-P (Ac3GlcNDAz-1-P(Ac-SATE)2) is prepared synthetically and added to cultured cells, which remove the protecting groups. The deprotected compound is converted to UDP-GlcNDAz by the action of a mutant form of AGX1. OGT transfers GlcNDAz from UDP-GlcNDAz to substrate proteins.