Table 4.
Fluorescence scanner data of investigated slides
| Fλexc[a] | bare glass[b] | APTES[c] | APTES+5[I][d] | APTES+5[II][d] | 5[e] |
|---|---|---|---|---|---|
| A[f] | B[f] | C[f] | D[f] | E[f] | |
| av488 | 17 | 35 | 23 255 | 12 740 | 194 |
| SD488 | ±17 | ±23 | ±3803 | ±935 | ±82 |
| av532 | 12 | 11 | 7416 | 5395 | 72 |
| SD532 | ±10 | ±12 | ±991 | ±268 | ±38 |
[a]
Average (av) arbitrary fluorescence intensity per pixel and standard deviation (SD) for excitation at 488 and 532 nm.
[b]
Piranha-cleaned slide.
[c]
APTES-functionalized slide.
[d]
Two different APTES-functionalized slides reacted with 5.
[e]
Piranha-cleaned slide (without APTES), treated with 5 and subjected to normal washing/drying procedure.
[f]
Slide abbreviation.