Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Allergy Clin Immunol. 2012 Nov 22;131(6):1653–1662.e1. doi: 10.1016/j.jaci.2012.10.012

FIG 7.

FIG 7

DJ-1 differentially regulates FcεRI-mediated activation of SHP-1 and SHP-2 and, in turn, phosphorylation of LAT and Syk, respectively. (A) Representative Western images (upper panel) and activities (lower panel) of immunoprecipitated SHP-1 or SHP-2 in BMMCs. (B and C) Representative Western images of whole cell lysates from BMMCs with transfection of control siRNAs or siRNAs against SHP-1 or SHP-2 (B), or Syk (C) in WT or DJ-1 KO BMMCs. (D) Percent release of granules in DJ-1 KO BMMCs with transfection of siRNAs against SHP-1 or SHP-2 (upper panel) or overexpression of DNA plasmid for SHP-1 or SHP-2 (lower panel). (A and D) All values are the means ± SEM from three independent experiments: * P < 0.05.