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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Allergy Clin Immunol. 2012 Nov 22;131(6):1653–1662.e1. doi: 10.1016/j.jaci.2012.10.012

FIG 8.

FIG 8

Fyn, SHP-1, and SHP-2 activities are regulated directly by H2O2 and a proposed scheme for DJ-1 regulation of ROS and downstream signals. (A and B) Activities of immunoprecipitated Fyn, SHP-1, and SHP-2 from non-stimulated (NS) and antigen-stimulated (Ag) BMMCs were assayed in vitro with different concentrations of H2O2 for 15 min. Values (means ± SEM) are from three independent experiments: * P < 0.05. (C) Representative confocal images and Western blot images for co-immunoprecipitation of LAT and SHP-1 or Syk and SHP-2. (D) Proposed scheme: Absence of DJ-1 leads to excess ROS which inhibits SHP-1 and enhances phosphorylation of LAT and LAT-dependent signals while inhibiting Fyn and, via activation of SHP-2, Syk. Nevertheless, the enhancement of LAT phosphorylation is sufficient to ultimately augment mediator release in DJ-1-deficient BMMCs and PCA in DJ-1-deficient mice.