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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Invest Dermatol. 2012 Nov 29;133(4):890–898. doi: 10.1038/jid.2012.407

Fig. 2.

Fig. 2

Epidermal deletion of Gpr177 abrogates the induction of hair follicles. β-gal staining of the E13.5 (a) and E14.5 (b) K5-Cre; R26R embryos analyzes the effectiveness of Cre recombination in the epidermis and hair placode. Sections of the control and Gpr177K5 embryos were analyzed by co-immunostaining of Gpr177 and K14 (c-d) and hematoxylin/eosin staining (e-h) at E14.5 (c-f) and E17.5 (g-h). Whole mount in situ hybridization of the control (i, k, m, o) and Gpr177K5 (j, l, n, p) embryos reveals the expression of Edar (i-j), Bmp2 (k-l), Bmp4 (m-n) and Shh (o-p) at E14.5. Control genotype: Gpr177Fx/Fx or K5-Cre; Gpr177Fx/+. Scale bars, 50 μm (a-h); 200 μm (i-p).