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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Invest Dermatol. 2012 Nov 29;133(4):890–898. doi: 10.1038/jid.2012.407

Fig. 5.

Fig. 5

The Gpr177-mediated regulation of Wnt in the dermis is dispensable for hair follicle initiation. β-gal staining of the E14.5 control and Gpr177Dermo1 skins carrying R26R shows Cre effectiveness (a-b). Sections are analyzed by co-labeling of Gpr177 and K14 (c-f) or CD133 and AE3 (q-r), H&E (g-h), immunostaining of Cadherin (i-l) and ABC (s-t) and in situ hybridization of Edar (m-n) and Shh (o-p), and β-gal staining of the Axin2lacZ allele (u-x) at E13.5, E14.5 and E15.5. (y) Graph indicates quantitative analysis for the placode region and the expression domain for ABC, Edar and Shh. Control genotype: Gpr177Fx/Fx or Dermo1-Cre; Gpr177Fx/+. Scale bars, 50 μm (a-x). (z) Model for epidermal Wnt in orchestrating signaling interaction between the epidermis and dermis during hair follicle development.