Skip to main content
. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Immunol. 2013 Feb 1;190(6):2756–2766. doi: 10.4049/jimmunol.1202697

Figure 3. Macrophages from XID and WT mice are not different in their interaction with SchuS4.

Figure 3

Macrophages were collected by bronchoalveolar lavage (alveolar) or differentiated from the bone marrow (Bone Marrow) and infected with SchuS4 at a MOI = 10. (A) At the indicated time points cells were lysed and intracellular bacteria were enumerated by plating lysates on MMH agar. (B) Twenty-four hours after infection cells were treated with Pam3CSK4. Supernatants were harvested 12 hours later and assessed for TNF-α by ELISA. Untreated, uninfected cells served as negative controls. Uninfected cells treated with Pam3CSK4 served as positive controls. *= significantly less than uninfected, Pam3CSk4 treated cells (p<0.05). Error bars represent SEM. Data is representative of three experiments similar design.