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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Immunol. 2013 Feb 1;190(6):2756–2766. doi: 10.4049/jimmunol.1202697

Figure 5. Control of SchuS4 infection in XID mice is correlated with fewer IL-10+ B1a cells and increased IL-12+ macrophages.

Figure 5

Mice (n=4–5/group) were intranasally infected with 50 CFU F. tularensis strain SchuS4. As indicated, animals received 5 mg/kg levofloxacin on days 3–6 of infection. Animals were euthanized on day 7 of infection and single cell suspensions of the lung and spleen were generated. Cells were incubated with PMA, ionomycin and BFA for 4 hours were stained for specific surface receptors, permeabilized and stained for IL-10 and IL-12 and assessed for specific cell populations by flow cytometry. B1a cells were characterized as CD5Y/CD19Y. Macrophages were characterized as CD11b+/F480Y. Error bars represent SEM. * = significantly greater than uninfected XID (p<0.05). ** = significantly greater than uninfected WT and all XID (p<0.05). *** = significantly greater than all other groups (p<0.05). Data is representative of two experiments of similar design.