Figure 1.

RANTES-induced transendothelial migration of T cells. (A) Spontaneous migration was stimulated when HLMVECs were pre-activated with IFN-γ plus TNF-α for 24 h, or when thrombin (1 U·mL⁻¹)-activated platelets (10⁸ mL⁻¹) were added basally 30 min before the assay (hatched bar in panel A, control conditions in panels B and C) and inhibited by a RANTES-neutralizing antibody (10 μg·mL⁻¹). Results are presented as mean ± SEM, n = 3, * P < 0.05, ** P < 0.01 and *** P < 0.001. (B) The copper chelators, neocuproine (NC) and bathocuproine disodium salt (BCDS), added to both the apical and basal wells 24 h before the assay inhibited T-cell migration. Results are presented as mean ± SEM, n = 3. (C) d-penicillamine and tobramycin added to both the apical and basal wells 24 h before the assay inhibited T-cell migration. Results are presented as mean ± SEM, n = 5.