Figure 5.

The effect of tobramycin and CuT on TNF-α-activated intracellular ROS generation in HLMVEC. (A) CLSM images of untreated HLMVEC (1), cells treated with 10 ng·mL⁻¹ TNF-α alone (5), and with 10 ng·mL⁻¹ TNF-α following treatment for 3 h with 0.01 mM Tob (2), 0.1 mM Tob (3), 0.5 mM Tob (4), 0.01 mM Cu-Tob (6), 0.1 mM Cu-Tob (7), 0.5 mM Cu-Tob (8). Scale bar 100 μm. (B) A calibration of total fluorescent intensity. (C) Fluorescent intensity for each condition analysed as the mean of at least four fields of view. The data expressed as mean ± SEM was analysed using one-way anova and Dunnett's post hoc test versus the TNF-α positive control, where * P < 0.05, **P < 0.01, ***P < 0.001. Differences between the effects of tobramycin and CuT were analysed using Student's two-tailed unpaired t-test with ^##P < 0.01 and ^###P < 0.001. Representative confocal images from a single experiment, representative of at least four independent experiments.