Figure 6.

The effect of tobramycin, CuT and SOD on superoxide release from OPZ-activated neutrophils. Neutrophils at 10⁷ mL⁻¹ suspended in HBSS with Ca/Mg + 20 mM HEPES, pH 7.4 were treated with OPZ alone and OPZ in the presence of a range of tobramycin, CuT and SOD concentrations added at the same time as freshly prepared OPZ (1 mg·mL⁻¹) for 15 min at 37°C with gentle shaking. Superoxide ions were measured by the reduction of cytochrome C (n = 3, independent experiments) at 550 nm. C is the spontaneous generation of O₂⁻ and OPZ is the generation of O₂⁻ by OPZ alone. The effect of the drugs was compared with OPZ alone. Data are represented as mean ± SEM. * P < 0.05, **P < 0.01, ***P < 0.001.