Table 1.
The effect of antioxidants on neutrophil TEM
| Inhibitor | Drug concentration | Inhibition of control [%] | n | Significance | Inhibition of control [%] | n | Significance |
|---|---|---|---|---|---|---|---|
| 30 min pretreatment | 16 h pretreatment | ||||||
| SOD | 2000 U·mL−1 | −10.8 ± 16.5 | 3 | ns | 14.6 ± 2.6 | 2 | ns |
| Catalase | 250 U·mL−1 | 15.3 ± 10.2 | 4 | ns | 32.2 ± 3.4 | 3 | P < 0.05 |
| SOD + Catalase | 2000 U·mL−1, 250 U·mL−1 | 25.7 ± 11.0 | 4 | P < 0.05 | |||
| MnTBAP | 50 μM | 43.5 ± 15.3 | 3 | P < 0.05 | 37.4 ± 5.7 | 3 | P < 0.05 |
| Tob | 0.01 mM | 19.4 ± 32.6 | 3 | ns | 20.3 ± 3.6 | 5 | P < 0.01 |
| Tob | 0.1 mM | 18.9 ± 8.4 | 3 | ns | 12.3 ± 5.2 | 5 | P < 0.05 |
| Tob | 0.5 mM | −8.8 ± 20.6 | 3 | ns | 10.1 ± 2.8 | 5 | ns |
| CuT | 0.01 mM | −5.0 ± 13.8 | 4 | ns | 3.2 ± 7.9 | 5 | ns |
| CuT | 0.1 mM | 11.6 ± 9.3 | 4 | ns | 16.3 ± 6.8 | 5 | ns |
| CuT | 0.5 mM | 19.1 ± 7.6 | 4 | ns | 33.8 ± 10.4 | 5 | P < 0.05 |
The treatment was compared with neutrophil (2 × 106 cells·mL−1) migration through TNF-α (10 ng mL−1)-stimulated HLMVEC towards thrombin (2 U·mL−1)-activated platelets (1 × 108 cells·mL−1). Inhibitors were added to upper and lower wells for 30 min or 16 h prior to TEM for 3 h. The data are expressed as mean ± SEM for at least three independent experiments. Data were analysed using two-tailed, paired t-test and one-way ANOVA with Dunnett's multiple comparison test, as appropriate.