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. 2013 Feb 20;168(5):1255–1265. doi: 10.1111/bph.12016

Figure 4.

Figure 4

eNOS-dependent, ACh-induced cGMP accumulation in adult mouse aortic rings. Rings were pre-incubated with the non-selective PDE inhibitor IBMX (1 mM) for 20 min. In slices from wild-type (eNOS+/+) mice, ACh (10 μM, 1 min) generated a significant increase in cGMP from basal (anova with Tukey–Kramer test, P < 0.001) that was abolished by the non-selective NOS inhibitor, l-NNA (100 μM, 30 min pretreatment; P > 0.05 compared with basal). The response to ACh was absent in slices from eNOS−/− mice (anova with Tukey–Kramer test, ACh vs. basal or L-NNA: P > 0.05). The response of eNOS+/+ and eNOS−/− slices to the NO donor, DEA/NO (100 μM, 5 min), did not differ significantly (unpaired t-test, P > 0.05). Numbers above bars are n-values; note the break in the y-axis.