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. 2013 Mar 7;49(5):858–871. doi: 10.1016/j.molcel.2013.01.002

Figure 4.

Figure 4

A Role for RIF1 in the Cellular Responses to DSBs

(A) WT and Rif1−/− MEFs were untreated (CNTL) or harvested at indicated times following γ-irradiation before immunostaining with the indicated antisera. Zoom panels correspond to indicated cells in left panels.

(B) γH2AX IRIF were scored in cells of indicated genotype 24 hr following mock treatment or IR at the indicated doses. Where indicated, cells were either mock treated (DMSO) or incubated with ATMi (KU55933, 10 μM) from 30 min before irradiation until harvesting. Mean number of γH2AX IRIF per cell is plotted ± SEM. n > 200 cells/condition over two independent experiments.

(C) The survival of MEFs of indicated genotype following control or γ-irradiation treatments was assessed by colony survival assay. n = 3 ± SEM.

(D) Measurement of NHEJ proficiency in HEK239 EJ5 cells subjected to the indicated siRNAs. n = 3 ± SEM unpaired two-tailed t test. See also Figure S5.