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. 2013 Mar 7;49(5):959–971. doi: 10.1016/j.molcel.2012.12.022

Figure 3.

Figure 3

Bax and Bcl-XL Can Stabilize Each Other on Mitochondria

(A) MECs stably expressing GFP-Bax were transfected with expression vectors for either mRFP-Bcl-XL or mRFP-Mcl-1. Eighteen hours post transfection, FLIP analysis was carried out as in Figure 1A in the presence or absence of ABT-737. The three graphs shown are from the same experiment, and the data for GFP-Bax (green line) are the same in all three plots. The bar graph on the left indicates that mRFP-Bcl-XL and mRFP-Mcl-1 inhibited detachment-induced apoptosis. Error bars represent SEM. See Figure S2.

(B) FRAP analysis of Bcl-XL and DsRed-Mito. MECs transiently expressing GFP-BclXL and DsRed-Mito were photobleached in the yellow ROI and imaged every 5 s. Fluorescence intensity was analyzed within the ROI and normalized to 100%. Error bars represent SEM. See Movie S6.

(C) Bax−/−Bak−/− DKO MEFs transiently expressing mRFP-Bcl-XL, either alone or with GFP-Bax, were analyzed by FRAP as in (B). Error bars represent SEM. See Movie S7.