Figure 4.

STAT1 activation in hCAF1 knockdown cells. (A) After IFN stimulation for the indicated times, the level of tyrosine 701 phosphorylation of STAT1 was measured in hCAF1^kd cells and control cells by western blot. (B, C) Kinetic induction of STAT1 and STAT1-target genes upon interferon stimulation for the indicated times in (B) hCAF1 knockdown and control cells and in (C) MCF7 cells stably expressing mCAF1 and control cells was analysed using quantitative PCR as described in Figure 1. The illustrated experiments were performed in triplicate, expressed as mean values of three independent experiments. Standard deviations are shown and the P-value was determined by Student’s t-test: *P<0.05; ***P<0.001. (D) Confocal fluorescence microscopy experiments showing the subcellular distribution of endogenous STAT1 and p-STAT1 in hCAF1-depleted cells. Mock and hCAF1^kd cells were grown on coverslips into 12-well plates and treated with 5 ng/ml of IFNγ for 1 h. STAT1 and p-STAT1 were analysed by immunofluorescence. Scale bar=20 μm.

Source data for this figure is available on the online supplementary information page.

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