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. 2013 Mar 12;3:1420. doi: 10.1038/srep01420

Figure 5. In vivo analysis of transplanted X-MET engineered muscle construct.

Figure 5

(a) Histochemical and immunohistological analysis of a transversal section of the transplanted area. Left panel: hAP stain (blue-violet coloration) shows fibers of transplanted X-MET. Middle panel: immunofluorescence analysis for embryonic myosin heavy chain (e-MyHC). hAP positive cells express also e-MyHC, revealing the donor origin of these cells. Right panel: immunofluorescence analysis for e-MyHC (red), laminin (green), and nuclei (blue). X-MET shows a histological continuity with the larger myofibers of recipient origin (at the top of panel). Scale bar, 100 μm. (b) Immunofluorescence for CD31 expression reveals the presence of endothelial structures within the area were X-MET was transplanted. Hoechst was used to stain nuclei (blue). Scale bar, 100 μm.