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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Growth Factors. 2012 Sep 5;30(5):320–332. doi: 10.3109/08977194.2012.717076

Figure 2.

Figure 2

KGF reduces TER of polarized Caco-2 cells and is detected in 18Co CM. (A) KGF (10 ng/ml), HGF (40 ng/ml), TGF-β1 (2 ng/ml), and IGF-2 (20 ng/ml) were added to polarized Caco-2 cells seven days after plating. Only KGF selectively reduced TER. Data shown are mean ± S.E. of three independent experiments. *p < 0.05, compared to TER in CTL medium. (B) CM from polarized Caco-2 cells, 18Co cells, or co-cultured Caco-2/18Co cells grown in 5% serum or serum-free culture conditions was collected and analyzed by ELISA for KGF. KGF was detected in 18Co CM and Caco-2/18Co CM, but not in Caco-2 CM. Data shown are mean ± S.E. of three independent experiments. *p < 0.001, compared to that in serum-free Caco-2 CM.