Table 3.
Effect of temperature during seed development and maturation on nucleotide content, mitochondrial respiration rate and adenylate energy charge (AEC) of excised wheat embryos after 4 h imbibition (adapted from Grass & Burris 1995)
| Temperature regime* | |||
|---|---|---|---|
| 20/15 °C | 28/21 °C | 36/29 °C | |
| Nucleotide content† (nmol/g) | |||
| AMP | 400 | 370 | 448 |
| ADP | 571 | 518 | 412 |
| ATP | 452 | 393 | 141 |
| Total | 1422 | 1180 | 1001 |
| State 3 mitochondrial respiration rate (nmol O2/min) | 9·7 | 6·9 | 5·3 |
| AEC‡ | 0·52 | 0·51 | 0·35 |
Day/night with 8 h day temperature and 16 h night temperature.
AMP, adenosine monophosphate; ADP, adenosine diphosphate; ATP, adenosine triphosphate.
AEC expressed as the ratio (ATP+0·5ADP/ATP+ADP+AMP)=energy status of the seed (Atkinson 1968).