Fig. 2.

Mutant α-synuclein over-expression enhances the affects of combined iron-paraquat-induced apoptosis in a dopaminergic cell line. α-Synuclein transfected N27 cells were treated with 350 µM paraquat and 80 µM FeCl₂ for 24 h. Cells were treated with EUK-189 1 h prior to the addition of FeCl₂ and paraquat. (a) TUNEL-positive cells were measured at 24 h. (b) Cell viability was measured via the MTT assay and expressed as a percentage of viability in control at 24 h. Gray bars, saline; white bars, paraquat alone; and black bars, paraquat plus FeCl₂. Mean ± SEM, n = 5. #p < 0.01, significantly different from vector plus saline plus saline; ##p < 0.05, significantly different from vector plus saline plus paraquat; ^p < 0.05, significantly different from matched vector plus saline; *p < 0.05, significantly different from A53T plus saline plus saline; **p < 0.05, significantly different from A53T plus saline plus paraquat; and @p < 0.01, significantly different from matched A53T plus saline. V + S, vector plus saline; V + EUK, vector plus EUK-189; A + S, A53T plus saline; A + E, A53T plus EUK-189.