Fig. 7.
Administration of EUK-189 prevents α-synuclein nitration in the SNpc. (a) Nitrated α-synuclein-immunopositive staining (green) within dopaminergic neurons (red). 4′,6-Diamidino-2-phenylindole (blue) was used to counter-stain nuclei. Scale bar, 20 µm. (b) Quantitative analysis of double labeling for TH with nitrated α-synuclein in the SNpc of 12-month-old A53T expressing transgenics. White bars, vehicle and black bars, EUK-189-treated. Mean ± SEM, the number of animal per group is 3 or 4. *p < 0.001, significantly different from saline plus saline plus vehicle group; @p < 0.05, significantly different from saline plus paraquat plus vehicle group; #p < 0.001, significantly different from matched vehicle group. S + S, saline plus saline; Fe + S, iron plus saline; S + PQ, saline plus paraquat; Fe + PQ, iron plus paraquat.