Fig. 3.

HeLa cells or A549 cells were incubated with cell culture medium or transiently transfected with either vector alone or a viperin overexpression construct. One day later, cells were mock treated or incubated with rgRSV at an MOI = 1. Two days later, HeLa or A549 cells were trypsinized and the percentage of infected cells was determined via flow cytometry. Representative flow-cytometric plots of the mean percentage of GFP-positive HeLa cells for each of the conditions tested are shown (a–d). e, f Percentages of GFP-positive cells (n = 3; e) and GFP-positive A549 cells (n = 3; f; means ± SD). * p ≤ 0.05, GFP-positive cells vs. cells with rgRSV alone. Overexpression of viperin resulted in an approximate 20–30% decrease in the percentage of RSV-infected epithelial cells.