Fig. 5.

Chinchillas (n = 4/cohort) were mock treated or inoculated with either rAAV that expressed GFP or viperin; 7 days later, animals were challenged with 1 × 10⁸ pfu RSV A2. After 7 days, animals were sacrificed, the nasal cavity embedded and sections of tissue from the ethmoid turbinates were incubated with a monoclonal antibody directed against anti-mouse viperin (a) followed by a secondary anti-mouse antibody conjugated to AlexaFluor 594 (red) and counterstained with DAPI to stain DNA (blue). As a comparison, we also evaluated viperin expression in archived embedded sections of ethmoid turbinate tissue obtained from naive chinchillas. b ImageJ densitometry analysis of sections presented in a showing the relative expression of viperin among the cohorts with the naive animals set to a value of zero. Two images from 3 animals per cohort were analyzed. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.0001, vs. naïve animals.