Figure 8.

Effect of the concentration of [³H]quinuclidinylbenzilate and the time of incubation on the binding of gallamine to solubilized M₂ receptor from Sf9 cells. Gallamine and [³H]QNB were added simultaneously to aliquots of the solubilized extract, and total binding was measured after incubation of the reaction mixture at 30 °C for different times as follows: 6 h (■, ●) and 12 h (□,○) (A), 30 h (■, ●) and 40 h (□,○) (B). The concentrations of [³H]QNB and the corresponding levels of occupancy were as follows: 3.9 nM, 54% (■,□); 22 nM, 87 % (●,○). The lines represent the best fit of Equation 3 (n = 2) to the pooled data at each level of occupancy, and the parametric values are listed in Table 6.