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. Author manuscript; available in PMC: 2014 Mar 6.
Published in final edited form as: Neuron. 2013 Mar 6;77(5):955–968. doi: 10.1016/j.neuron.2012.12.038

Figure 3. GCs in the dorsal but not ventral DG selectively control the encoding of contextual fear memories.

Figure 3

(A–B) Yellow light illumination of the dorsal DG during training blocked the encoding of context fear as POMC-eNpHR3.0 mice exhibited reduced freezing when tested 24hr later in the absence of light (n=6–7/geno, repeated measures ANOVA, genotype effect F(1,11)=13.2, p=0.004, genotypeXtraining interaction F(1,11)=9.4, p=0.01 (t11=3.4, p=0.006) (C) Optogenetic inhibition of the dorsal DG selectively impairs the encoding of contextual fear memories, as yellow light illumination of the dorsal DG during a recall session did not impair the retrieval of contextual fear memories. (n=7–8/geno, repeated measures ANOVA, geno effect F(1,13)= 0.19, p=0.7, training effect F(1,13)= 42.7, p=<0.0001, genotype X training interaction F(1,13)= 0.19, p=0.7 (t13=−.4, p=0.7) (D–E) POMC-eNpHR3.0 mice received yellow light stimulation of the ventral DG during encoding, were tested and retrained in the absence of light, then tested for light effects on retrieval. (E) Optogenetic inhibition of the ventral DG had no impact in acquisition or retrieval of contextual fear memories. (n=6/geno light on during encoding, repeated measures ANOVA, geno effect F(1,10)= 0.01, p=0.9, training effect F(1,10)= 19.5, p<0.01, genotype X training interaction F(1,10)= 0.03, p=0.9, light on during retrieval, geno effect F(1,10)= 0.1, p=0.8, genotype X training interaction F(1,10)= 0.05, p=0.8, (t10=−.3, p=0.8). **p<0.01. All error bars are +/− SEM.