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. Author manuscript; available in PMC: 2014 Mar 6.
Published in final edited form as: Neuron. 2013 Mar 6;77(5):955–968. doi: 10.1016/j.neuron.2012.12.038

Figure 4. Optogenetic inhibition of the dorsal DG impairs discrimination in an active place avoidance task.

Figure 4

(A) Experimental design. Shown are the last training trial and the conflict trial. (B) Optogenetic inhibition of the dorsal DG did not impact exploration of the apparatus in either training or conflict trial phases (n=5–6/geno, repeated measures ANOVA, geno effect F(1,9)= 1.1, p=0.3, training effect F(1,9)= 1.3, p=0.3, genotype X training interaction F(1,9)= 2.7, p=0.1 (C) Time-in-location heat maps for POMC-eNpHR3.0 and control mice during the first 20 min of the last training trial and the 20min conflict trial. (D) Percent time in each of the zones during the training trial and the conflict trial. In training, a slightly different strategy was used, but both groups effectively avoided the shock zone (genotype X training interaction F(5,45)= 5.4, p<0.01, t-test on zone D, t9=−3.4, p<0.05). In the conflict trial, POMC-eNpHR3.0 mice spent significantly less time in the zone opposite the shock zone and more time adjacent to the shock zone (genotype X training interaction F(5,45)= 2.5, p<0.05, t-test on zone D, t9=4.1, p<0.05, zone C, t9=−2.3, p<0.05) (D–E) As a consequence, POMC-eNpHR3.0 mice exhibited an increased number of entrances into the shock zone after switching its location (unpaired t test, t9=−2.3, p<0.05, and the percent time in the quadrant opposite the new shock zone (t9=3.4, p<0.01). **p<0.01, *p<0.05. All error bars are +/− SEM.