Figure 1.

Inserted. Effect of BaV on cell viability. Thioglycollate-elicited macrophages were collected 96hr after i.p. injection of thioglycollate. Cells (2x10⁵) were incubated with different concentrations of BaV or RPMI (control) for 60min at 37°C under 5% (v/v) CO₂, after which cytotoxicity was assessed by Trypan blue exclusion. Values represent the mean ±SEM from four animals. *p<0.05 compared to controls (ANOVA). A. Phagocytosis of opsonized zymosan particles by thioglycollate-elicited macrophages. Cells were harvested 96hr after i.p. injection of thioglycollate and were incubated with BaV or RPMI (control) for 60min before addition of opsonized zymosan particles. Values represent the mean ±SEM from four animals. *p<0.05 compared to controls (ANOVA). B. Effect of BaV on superoxide anion production by migrated leukocytes. The cells were collected from mice peritoneal cavity 6hr after BaV (0.2mg/kg) or sterile saline alone (0.15M NaCl) (control) injection. 2x10⁵cells/ml were incubated for 40min with NBT. Cells were then stained with safranin and centrifugated (400xg/5min) in cytospin. NBT positive cells were evaluated by cell count crystals that formed inside of the cell by phase contrast microscope. Values represent the mean ±SEM of 4 animals. *p<0.01 compared to control (ANOVA).