. Author manuscript; available in PMC: 2013 Sep 5.

Published in final edited form as: Nat Commun. 2013;4:1547. doi: 10.1038/ncomms2461

Table 1.

Rat number and correlation of Hcrt and MCH levels in the hypothalamus and amygdala.

	Correlation of Hcrt in Hypothalamus and Amygdala			Correlation of MCH in Hypothalamus and Amygdala
	Number of XY Pairs	R Value	P Value	Number of XY Pairs	R Value	P Value
Rat 1	235	0.6575*	<0.0001	219	0.2372*	<0.0004
Rat 2	244	0.7264*	<0.0001	243	0.6909*	<0.0001
Rat 3	246	0.5924*	<0.0001	246	0.4904*	<0.0001
Rat 4	256	0.4982*	<0.0001	256	0.2757*	<0.0001

Statistical analysis using Pearson product-moment correlation coefficient.

In all rats, Hcrt levels in the hypothalamus were significantly correlated with levels in the amygdala. MCH levels in the hypothalamus were also significantly correlated with MCH levels in the amygdala. Potential sources of variability in peptide level assessments include differences in placement of electrode within target region, protein deposition on the dialysis membrane and noise in RIA measurement of peptide levels.