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. Author manuscript; available in PMC: 2013 Jul 6.
Published in final edited form as: Cell. 2012 Jul 6;150(1):179–193. doi: 10.1016/j.cell.2012.05.038

Figure 4. Acetylation of Skp2 Positively Regulates Skp2 Oncogenic Functions.

Figure 4

(A) IB analysis of LNCaP cell lines stably transfected with the indicated HA-Skp2 constructs.

(B) Cell growth curves of the various LNCaP cell lines stably expressing the indicated HA-Skp2 constructs. Results were presented as mean ±SD from three independent experiments.

(C) Various LNCaP cell lines stably expressing the indicated HA-Skp2 constructs were pulsed with BrdU for 30 min, and the BrdU incorporation rate was measured. Results were presented as mean ±SD from three independent experiments.

(D and E) LNCaP cells stably transfected with the indicated HA-Skp2 constructs (with empty vector as a negative control) were injected subcutaneously into nude mice (n = 5 for each group) and examined over time for in vivo tumorigenesis. Pictures in (E) were taken 6 weeks after injection. Results were presented as mean ±SD and *p < 0.05 (Student’s t test).

See also Figure S4.