(A) IB analysis of LNCaP cell lines stably transfected with the indicated HA-Skp2 constructs.
(B) Cell growth curves of the various LNCaP cell lines stably expressing the indicated HA-Skp2 constructs. Results were presented as mean ±SD from three independent experiments.
(C) Various LNCaP cell lines stably expressing the indicated HA-Skp2 constructs were pulsed with BrdU for 30 min, and the BrdU incorporation rate was measured. Results were presented as mean ±SD from three independent experiments.
(D and E) LNCaP cells stably transfected with the indicated HA-Skp2 constructs (with empty vector as a negative control) were injected subcutaneously into nude mice (n = 5 for each group) and examined over time for in vivo tumorigenesis. Pictures in (E) were taken 6 weeks after injection. Results were presented as mean ±SD and *p < 0.05 (Student’s t test).
See also Figure S4.