Figure 1. Val-boroPro induces complete regression in multiple tumor models and requires inhibition of intracellular PPCE.

(A) C57BL/6 female mice were inoculated with 1×10⁶ MB49 on day 0. Mice were treated with saline (open squares) or 20 µg Val-boroPro 5×/week during weeks 1 through 4 (days 3–28, closed squares), week 1 only (days 3–7, open triangles), or weeks 2 through 4 (days 10–28, inverted solid triangles) (n = 6/group). All treatment groups are statistically different than saline (Anova, p<0.05 for late treament vs saline and p<0.01 for early treatment groups vs saline). The days 10–28 group is also statistically different than both early treatment groups (p<0.05). (B) C57BL/6 mice were inoculated with 1×10⁶ M3-9-M on day 0 and treated with 20 ug Val-boroPro (dashed line) or saline (solid line) for four weeks (n = 5/group). Tumor volumes are statistically different between the Val-boroPro and saline groups at p<0.001 for all times beyond day 10. (C) Mice were inoculated with MB49 as in Figure 1A and treated with 20 ug Val-boroPro (open squares), 20 ug PT-630 (open triangles), or saline (closed squares) (n = 5/group). (D) Mice were inoculated with M3-9-M as in Figure 1B and treated with PT-630 (open triangles) or saline closed squares) (n = 5/group). Mean tumor volumes show that PT-630 treatment was no different from saline. Figures 1A–D are representative of 3 or more experiments.