Figure 10. Val-boroPro is an effective adjuvant to tumor primed dendritic cell vaccination.

(A) C57BL/6 female mice were challenged with MB49 (1×10⁶) on day 0 and treated with 20 µg Val-boroPro or saline from days 3–7 (closed squares) or 10–14 (n = 5/group). On day 12, late-treatment groups received either a male-derived HY-expressing DC vaccine or a female-derived DC vaccine IP (1×10⁵). Tumors were significantly smaller in mice receiving male DC vaccine plus Val-boroPro during week 2 (open circles) compared to no treatment (open squares), male DC alone (closed triangles) or female DC plus Vale-boroPro during week 2 (closed circles) (p<0.01, Anova with Tukey post-test) (B) C57BL/6 mice were injected intramuscularly with M3-9-M and treated with Val-boroPro starting on day 10. Bone marrow-derived DCs pulsed with irradiated M3-9-M were given IP on day 12 (n = 8/group). Tumors were significantly smaller in mice receiving combined treatment (open triangles) compared to no treatment (closed squares) or either treatment alone (open squares or closed triangles) (p<0.01, Anova). Figures 7A and 7B are both representative of 2 experiments.