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. 2013 Mar 12;8(3):e58929. doi: 10.1371/journal.pone.0058929

Figure 2. Effect of miR-574-3p overexpression on PCa cell proliferation, migration and invasion in vitro and in vivo.

Figure 2

(A) miR-574-3p expression levels in PCa cell lines (PC3 and DU145) were determined by real-time PCR at 72 hours after transfection of Pre-miR miRNA precursor. miR-574-3p expression was normalized to RNU48. Data are presented as the mean ± SE. (B) Overexpression of miR-574-3p significantly inhibits cell viability. Cell viability was analyzed by the MTS cell proliferation assay 1, 2 and 4 days after transient transfection. *, P<0.05. (C) Over-expression of miR-574-3p significantly inhibits cell migration. After transfection (48 hours), a wound was formed by scraping and measured after 6, 12 and 24 hours. Representative images of wound healing assay are shown at 200× magnification. **, P<0.0001. *, P<0.005. (D) Over-expression of miR-574-3p significantly decreased cell invasion. Representative images of invasion assay are shown at 200× magnification. *, P<0.005. (E) Representative images of tumors in nude mice 5 weeks after subcutaneous injection of transfected miR-574-3p DU145 cell lines or control cell lines and time course of tumor growth.