Skip to main content
. 2013 Mar 12;8(3):e58929. doi: 10.1371/journal.pone.0058929

Figure 4. miR-574-3p targets RAC1, EGFR, EP300.

Figure 4

(A) Putative miR-574-3p binding and mutated sites in the 3′UTR of target genes. (B) Luciferase reporter assays using vectors encoding putative 3′UTR binding sites. PC3 and DU145 cells were transiently transfected with Pre-miR miRNA precursor or negative control, followed by transient transfection with basic vector or wild-type 3′UTR reporter plasmids or mutated 3′UTR plasmids for 24 hours. 3′UTR reporter activity was measured by luciferase assay and normalized to the activity of Renilla luciferase. Data are presented as the mean ± SE. *, P<0.05. (C). The mRNA levels of the three target genes of miR-574-3p were determined by quantitative real-time PCR analyses after transfection with miR-574-3p mimics and negative control in PCa cell lines (PC3 and DU145). *, P<0.05. (D) Immunoblot analysis for target genes in miR-control and miR-574-3p transfected PC3 cells. GAPDH was used as a loading control.