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. 2013 Mar 12;8(3):e59034. doi: 10.1371/journal.pone.0059034

Figure 5. Quantitative Real Time PCR of generated HMS174(DE3) mutant strains.

Figure 5

(A) The generated mutant strains HMS174(DE3)ΔinsAB lacUV5-flhDC, HMS174(DE3)ΔinsAB lacUV5-flhDC, att7 lacUV5-flhDC, HMS174(DE3) ΔinsAB T7-flhDC and HMS174(DE3)LT7 were incubated for 1 h after induction with 1 mM IPTG at 37°C/225 rpm. Quantification of flhD mRNA levels was accomplished with the comparative ΔΔCt method using rpoD for normalization. Resulting data were analyzed via 2-tailed type 2 Student's t-test, ***p<0.001, n = number of replicates.