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. 2013 Mar 12;8(3):e58015. doi: 10.1371/journal.pone.0058015

Figure 1. SUB1 is required for repair of dsDNA breaks in plasmid DNA.

Figure 1

Repair efficiency is the ratio of the number of stable transformants obtained when transformed with linearized versus circular pRS315 plasmid DNA. Data are normalized to the ligation efficiency of wild type. Asterisks * indicate a significant difference from wild type (P<0.05). All strains are derivatives of the wild type (MVY101), sub1Δ (MVY105), yku70 (MVY601), sub1Δ yku70 (MVY603). A. Repair efficiencies of the BamHI-linearized plasmid. BamHI produces a unique DSB in pRS315 with 5' end overhangs. B. Repair efficiencies of the PstI-linearized plasmid. PstI produces a unique DSB in pRS315 with 3' end overhangs. C. Repair efficiencies of the SmaI-linearized plasmid. SmaI produces a unique DSB in pRS315 with blunt DNA ends.