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. 2013 Mar 12;8(3):e58015. doi: 10.1371/journal.pone.0058015

Figure 2. Ligation accuracy of the plasmid repair.

Figure 2

. Strains, same as in Figure 1. A. Plasmid map of pMV1328. The unique NcoI restriction site within the KanMX6 gene is indicated. B. Repair efficiencies of the mutants when the NcoI linearized pMV1328 is used in the repair assay. Asterisks * indicate a significant difference from wild type (P<0.05). C. Accuracy of plasmid ligation is measured by testing Leu+transformants for G418 resistance (G418R), which requires accurate religation of the NcoI site within the KanMX6 gene.