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. 2013 Mar 12;8(3):e58015. doi: 10.1371/journal.pone.0058015

Figure 3. SUB1 is not required for repair of chromosomal breaks.

Figure 3

All strains used are derived from the ‘donorless’ strain JKM179 (MVY610): sub1Δ (MVY617), yku70 (MVY614), sub1Δ yku70 (MVY625). CFU: Colony Forming Units. A. Cell survival after HO induction for 3 hours followed by repression by glucose. B. Plasmid repair assay is performed in the JKM179 background. Repair efficiencies are normalized to wild type. C. Quantification of DSBs after HO induction. The yeast MATα locus is cleaved by HO endonuclease and the fraction of remaining, intact MATα DNA is determined by real time PCR. D. Cell survival when HO is constantly induced by galactose. Cells are plated on galactose-containing plates to count surviving cells and glucose-containing plates to count the total number of cells.