Figure 3. Relative contribution of NOS isoforms to basal NO production in BM of Lewis rats.

The basal NO level that was obtained in absence of a stimulus was taken as 100%. Contribution of the NOS isotypes was determined using two concentrations of the NOS isoform-specific inhibitor 1400 W. 1400 W was used at final concentrations of 0.1 µM for iNOS inhibition and 10 µM for inhibition of both iNOS and nNOS. With L-NAME (500 µM, final column), the signal intensity was below the detection threshold. Values represent biological triplicates.