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. 2013 Mar 12;8(3):e58342. doi: 10.1371/journal.pone.0058342

Figure 2. Effects of 5ML on blood vessel formation in the chicken chorioallantoic membrane assay (CAM) assay.

Figure 2

Figure 2A: CAMs of 3-days- old chickens were incubated for 96 hours with the control solution (DMSO as the solvent control) or 0.1 or 0.5 µg 5ML. Figure 2A shows that the treatment with 5ML enhanced the formation of new blood vessels. 5ML significantly increased blood vessel formation at a concentration of 0.5 µg/egg. Figure 2B shows representative images of the CAM-assay (Magnification: 12,5×). Blood vessels were counted in the ring area of the CAM to quantify effects of 5ML in vivo. All experiments were performed in triplicates. Asterisks indicate significant differences (* p<0.05) compared to the corresponding controls.