Skip to main content
. 2013 Mar 12;8(3):e58342. doi: 10.1371/journal.pone.0058342

Figure 3. 5ML alters the transcription of a small set of genes; CYP26B1 is the causal mediator of 5ML -induced angiogenesis.

Figure 3

Figures 3A and 3B show median values +/− S.D. of microarray-based analyses of gene-regulation by 5ML in relation to controls 6 and 24 hours after the addition of 5ML (10 µM). Figure 3C shows that despite the fact that the knockdown of CYP1A1 had a significant inhibitory effect on spontaneous angiogenesis in HUVECs; CYP1A1 knockdown had no effect on angiogenesis increased by 5ML. Similarly, CYP26B1 knockdown potently repressed spontaneous angiogenesis in HUVECS and in addition completely abrogated 5ML-induced tube formation. Shown are mean values of two independent experiments performed in triplicates +/− SD. * p<0.05. Figure 3D shows Western blot analyses of effects of the CYP1A1- and the CYP26B1-specific knockdown on protein expression of the corresponding proteins. Asterisks indicate significant differences (* p<0.05) compared to the corresponding controls.